Accepted Preprint (first posted online 7 June 2016)

    CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists

    1. Frederic Flamant
    1. S Markossian, Institut de Génomique fonctionnelle de Lyon, Ecole normale superieure de Lyon, Lyon, France
    2. F Flamant, Institut de Génomique fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France
    1. Correspondence: Frederic Flamant, Email: Frederic.Flamant{at}


    CRISPR/Cas9 is a recent addition to the mouse genetic toolbox . It allows generation of null, conditional, precisely mutated, reporter or tagged alleles in mice. Current progresses still broaden the outstanding possibilities that it provides for precise genome manipulations. We review here the strengths and limitations of the method which will soon give rise to a new generation of mouse models in endocrinology laboratories.

    • Received 1 February 2016
    • Revision received 3 June 2016
    • Accepted 7 June 2016
    • Accepted Preprint first posted online on 7 June 2016

    This Article

    1. J Mol Endocrinol JME-15-0305
    1. Abstract
    2. All Versions of this Article:
      1. JME-15-0305v1
      2. 57/2/R81 most recent

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