Long-term oral exposure to bisphenol A induces glucose intolerance and insulin resistance

    1. Kyong Soo Park1
    1. 1Department of Internal Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea
      2Department of Internal Medicine, Boramae Medical Center, Seoul 156-707, Republic of Korea
      3Department of Internal Medicine, Chung-Ang University Hospital, College of Medicine, Chung-Ang University, Seoul 156-755, Republic of Korea
      4Clinical Research Institute, Seoul National University Hospital, Seoul 110-74, Republic of Korea
      5Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Republic of Korea
    1. Correspondence should be addressed to Y J Park; Email: yjparkmd{at}snu.ac.kr


    Bisphenol A (BPA) is a widely used endocrine disruptor. Recent epidemiologic results have suggested an association between exposure to BPA and cardiovascular disease, type 2 diabetes, and obesity. We investigated the in vivo effects of long-term oral exposure to BPA on insulin resistance and glucose intolerance. In the present study, 4- to 6-week-old male mice on a high-fat diet (HFD) were treated with 50 μg/kg body weight per day of BPA orally for 12 weeks. Long-term oral exposure to BPA along with an HFD for 12 weeks induced glucose intolerance in growing male mice. Intraperitoneal glucose tolerance tests showed that the mice that received an HFD and BPA exhibited a significantly larger area under the curve than did those that received an HFD only (119.9±16.8 vs 97.9±18.2 mM/min, P=0.027). Body weight, percentage of white adipose tissue, and percentage of body fat did not differ between the two groups of mice. However, treatment with BPA reduced Akt phosphorylation at position Thr308 and GSK3β phosphorylation at position Ser9 in skeletal muscle. BPA tended to decrease serum adiponectin levels and to increase serum interleukin 6 and tumor necrosis factor α, although these findings were not statistically significant. Treatment with BPA did not induce any detrimental changes in the islet area or morphology or the insulin content of β cells. In conclusion, long-term oral exposure to BPA induced glucose intolerance and insulin resistance in growing mice. Decreased Akt phosphorylation in skeletal muscle by way of altered serum adipocytokine levels might be one mechanism by which BPA induces glucose intolerance.

    • Received in final form 29 April 2015
    • Accepted 13 May 2015
    • Made available online as an Accepted Preprint 13 May 2015
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