Supplementary Data

• Supplementary Figure 1 - Effects of graded doses of the aromatase inhibitor letrozole (0.25, 0.5, or 5 µM) on the accumulation of estradiol (E2) in cultures of fetal ovarian pieces over 10 days. Doses of letrozole were tested without (white bars) or with T+LH+FSH (black bars; testosterone, 0.5 µM; LH and FSH each at 100 ng/ml). Means with no common letters are different (P < 0.05). N = 6 cultures/treatment, 2 from each of 3 fetuses (86 to 89 days old). (PDF 15 KB)
• Supplementary Figure 2 - Accumulation of estradiol (E2) in cultures of fetal ovarian pieces over 10 days in control medium (Con) or with LH, FSH, or LH+FSH (100 ng/ml), each in the absence (open bars) or presence (black bars) of the aromatase inhibitor letrozole (Let; 0.25 µM). Means with no common letters are different (P < 0.05). N = 10 cultures/treatment, 2 from each of 5 fetuses (76 to 89 days old). (PDF 14 KB)