Journal of Molecular Endocrinology

Effects of an SGK1 inhibitor on intestinal SGLT1 expression in db/db mice. Mice were fed chow containing either no compound (db/m group, db/m; db/db group, db/db) or EMD638683 at 4.46 mg/g of chow (db/db+EMD group, db/db+EMD) from 10 weeks of age, and intestinal tissue was excised at 18 weeks of age and analyzed. (A and B) SGK1 mRNA (A) and protein (B) levels in intestinal tissue from db/db and control db/m mice. (C and D) SGLT1 mRNA (C) and protein (D) levels in intestinal tissue from db/m, db/db, and db/db+EMD mice. The total RNA was isolated from intestinal tissue, and first-strand cDNA was synthesized as described in the Methods section. Real-time quantitative PCR was performed, and the values were normalized to the level of β-actin mRNA. Protein was extracted from the intestinal tissue and subjected to western blot analysis. The relative protein levels were normalized to the GAPDH or β-actin protein level. The bands were quantified through densitometry using the Quantity One system. The results (means ± s.d.) are expressed as the fold change relative to the levels of the db/m group (Con, 100% or 1). ^#P<0.05 vs control group of db/m mice. *P<0.05; **P<0.01 vs control group in db/db mice.