Supplementary Data

• Supplementary Video 1 - Long term time lapse SPIM imaging of a Tg(fli1:EGFP)^y1 zebrafish embryo showing the development of the circulatory system in the yolk area. Images were acquired every 5 minutes for 3 days. A dataset consisting of 150 sections of the sample was acquired in each time point and is shown as Maximum Intensity Projection. The total measurement time of each time point was approximately 3 seconds. Images were acquired using a double sided illumination SPIM setup that incorporates a 473nm laser for excitation and a 4X microscope objective for fluorescence detection (Candeo, et al. 2016). The sample was anesthetized in tricaine and mounted vertically in the microscope (Bassi et al. 2015). (PDF 11220 KB)