Accepted Preprint (first posted online 5 June 2015)

    HORMONE MEASUREMENT GUIDELINES: Tracing lipid metabolism: the value of stable isotopes

    1. Margot Umpleby
    1. M Umpleby, United States
    1. Correspondence: Margot Umpleby, Email: m.umpleby{at}


    Labelling molecules with stable isotopes to create tracers has become a gold standard method to study the metabolism of lipids and lipoproteins in humans. There are a range of techniques which use stable isotopes to measure fatty acid flux and oxidation, hepatic fatty synthesis, cholesterol absorption and synthesis and lipoprotein metabolism in humans. Stable isotope tracers are safe to use, enabling repeated studies to be undertaken and allowing studies to be undertaken in children and pregnant women. This review provides details of the most appropriate tracers to use, the techniques which have been developed and validated for measuring different aspects of lipid metabolism and some of the limitations of the methodology.

    • Received 17 October 2014
    • Received in final form 13 May 2015
    • Accepted 4 June 2015
    • Accepted Preprint first posted online on 5 June 2015

    This Article

    1. J Endocrinol JOE-14-0610
    1. Abstract
    2. All Versions of this Article:
      1. JOE-14-0610v1
      2. 226/3/G1 most recent

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