Supplementary Data

• Supplementary Figure 1 - Schematic presentation of measurements performed in high-fat-fed Stk25 transgenic and wild-typemice. IPGTT, intraperitoneal glucose tolerance test; qRT-PCR, quantitative real-time PCR. (PDF 583 KB)
• Supplementary Figure 2 - Analysis of STK25 protein abundance in the pancreas of high-fat-fed Stk25 transgenic and wild-type mice. Representative immunofluorescence images stained with antibody for STK25; nuclei stained with DAPI(blue). Dashed lines denote islet boundaries. Scale bars, 15 μm. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 2018 KB)
• Supplementary Figure 3 - Measurement of mRNA expression in pancreatic islets isolated from high-fat-fed Stk25 transgenic and wild-type mice. Pancreatic islets were incubated overnight at 37°C in RPMI 1640 medium containing 11 mM glucose, followed by 60 min incubation in RPMI 1640 medium containing 5.5 mM glucose. Relative mRNA expression was assessed by quantitative real-time PCR. The expression level in wild-type mice is set to 1. Data are mean ± SEM from 4-5 mice per genotype. **p< 0.001. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 131 KB)
• Supplementary Figure 4 - Measurement of mRNA expression in pancreatic extracts of high-fat-fed Stk25 transgenic and wild-type mice. Relative mRNA expression was assessed by quantitative real-time PCR. The expression level of each gene in wild-type mice is set to 1. The gene functions are indicated at the bottom. Data are mean ± SEM from 7-12 mice per genotype. *p< 0.05. (PDF 124 KB)
• Supplementary Figure 5 - Measurement of mtDNA content in pancreatic extracts of high-fat-fed Stk25 transgenic and wild-type mice. Relative mtDNA content was calculated as the ratio of a mitochondrial-encoded gene (Coxl, forward 5′-ACTATACTACTACTAACAGACCG-3′, reverse 5′-GGTTCTTTTTTTCCGGAGTA-3′) to a nuclear-encoded gene (Cyclophilin A, forward 5′-ACACGCCATAATGGCACTGG-3′, reverse 5 ′-CAGTCTTGGCAGTGCAGAT-3′) DNA levels, determined by quantitative real-time PCR. The mtDNA content in wild-type mice is set to 1. Data are mean ± SEM from 10 mice per genotype. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 112 KB)
• Supplementary Figure 6 - Representative transmission electron microscopy images of the pancreatic islets of high-fat-fed Stk25 transgenic and wild-type mice. Scale bars, 1 μm. G, insulin granules; HFD, high-fat diet; M, mitochondria; TG, transgenic; WT, wild type. (PDF 2797 KB)
• Supplementary Figure 7 - Quantification of Ly6C staining in pancreatic islets of high-fat-fed Stk25 transgenic and wild-type mice. Data are mean ± SEM from 6 mice per genotype. *p<0.05. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 111 KB)
• Supplementary Figure 8 - Quantification of the number of vessels in the pancreatic tissue of high-fat-fed Stk25 transgenic and wild-type mice. A: Representative images stained with H-E. Scale bars, 50 μm. B: Quantification of the number of vessels. Data are mean ± SEM from 6 mice per genotype. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 2323 KB)
• Supplementary Figure 9 - C-peptide/insulin ratio assessed during IPGTT in high-fat-fed Stk25 transgenic and wild-type mice. The levels were measured in plasma collected during the experiment shown in Fig. 6. Data are mean ± SEM from 12-13 mice per genotype. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 94 KB)
• Supplementary Figure 10 - In vitro glucose-stimulated insulin secretion from pancreatic islets isolated from high-fat-fed Stk25 transgenic and wild-type mice. Data are mean ± SEM from 4-5 mice per genotype. HFD, high-fat diet; TG, transgenic; WT, wild-type. (PDF 93 KB)
• Supplementary Table 1 - List of antibodies used for immunofluorescence (PDF 115 KB)
• Supplementary Table 2 - Sequences of custom-designed primers (forward and reverse) and probes used for quantitative real-time PCR (PDF 157 KB)