In female rat heart mitochondria, oophorectomy results in loss of oxidative phosphorylation

    1. Salvador Uribe-Carvajal2
    1. 1Departamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, México, Mexico
    2. 2Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F., Mexico
    3. 3Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México, Mexico
    4. 4Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química UNAM, México D.F., Mexico
    5. 5División Académica Multidisciplinaria de Comalcalco, Universidad Juárez Autónoma de Tabasco, México, Mexico
    6. 6Departamento de Instrumentación Electromecánica, Instituto Nacional de Cardiología Ignacio Chávez, Tlalpan DF, México, Mexico
    7. 7Departamento de Consulta externa, Instituto Nacional de Cardiología Ignacio Chávez, México, Mexico
    1. Correspondence should be addressed to N Pavón; Email: pavitonat{at}


    Oophorectomy in adult rats affected cardiac mitochondrial function. Progression of mitochondrial alterations was assessed at one, two and three months after surgery: at one month, very slight changes were observed, which increased at two and three months. Gradual effects included decrease in the rates of oxygen consumption and in respiratory uncoupling in the presence of complex I substrates, as well as compromised Ca2+ buffering ability. Malondialdehyde concentration increased, whereas the ROS-detoxifying enzyme Mn2+ superoxide dismutase (MnSOD) and aconitase lost activity. In the mitochondrial respiratory chain, the concentration and activity of complex I and complex IV decreased. Among other mitochondrial enzymes and transporters, adenine nucleotide carrier and glutaminase decreased. 2-Oxoglutarate dehydrogenase and pyruvate dehydrogenase also decreased. Data strongly suggest that in the female rat heart, estrogen depletion leads to progressive, severe mitochondrial dysfunction.

    • Received 15 November 2016
    • Accepted 21 November 2016
    • Made available online as an Accepted Preprint 21 November 2016
    | Table of Contents