Supplementary Data

• Supplementary Figure 1 - Effect of embryonic treatment on survival and phenotypic abnormalities. The cumulative effects of treating larvae with dexamethasone (A), hypoxia (B) and GR Mo (C) are shown as the sum of normal criteria for each fish (i.e. a maximum 6 value represent normality). Data are analysed by One-way ANOVA and Bonferroni post-hoc analysis (n= 10). The appearances of fish were assessed according to six criteria (D and E). Panel F illustrates physical features affecting eye (E), swim bladder (SB), tail (T), heart (H) and yolk sac (YS) that were assessed; abnormalities were pin head (PH), small eye (SE), unusual side ballooning (USB), tail abnormalities (TA), heart (H), pericardial oedema (PO), yolk sac oedema (YSO), yolks sack extension oedema (YSEO). (PDF 186 KB)
• Supplementary Figure 2 - Glucocorticoid receptor morpholino knockdown. Head-trunk angle was assessed in larvae following co-injection of rescue gr mRNA (rescue) with GR Mo. Data shown are for larvae treated with GR Mo, GR Mo co-injected with rescue and Mo control. Data are mean (n=3 (6 larvae per group)) ±SEM were analysed by two-way ANOVA and Bonferroni post hoc test; *p≤0.05, **p≤0.01 and ***p≤0.001. (PDF 220 KB)
• Supplementary Table 1 - Primer sequences. Genes of interest with forward and reverse primer sequence used for qRT-PCR analysis of gene expression in larvae following manipulation with either the glucocorticoid receptor (GR) antagonist dexamethasone, the targeted knock-down of GR by morpholino (GR Mo) or exposure to the physical stressor hypoxia (5% O₂). (PDF 106 KB)