JOE Cross-Journal Searching
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]
Author:
Keyword(s):
Year:  Vol:  Page: 


This Article
Alert me when this article is cited
Alert me if a correction is posted
Services
Similar articles in this journal
Similar articles in ISI Web of Science
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Cited by other online articles
Search for citing articles in:
ISI Web of Science (341)
Google Scholar
Articles by Breier, B.
Articles by Gluckman, P.
Articles citing this Article
PubMed
PubMed Citation
Articles by Breier, B.
Articles by Gluckman, P.
Journal of Endocrinology, Vol 128, Issue 3, 347-357
Copyright © 1991 by Society for Endocrinology


Articles

Radioimmunoassay for insulin-like growth factor-I: solutions to some potential problems and pitfalls

BH Breier, BW Gallaher, and PD Gluckman


This report describes essential requirements for the validation of a radioimmunoassay (RIA) for insulin-like growth factor-I (IGF-I) and presents solutions to some problems and pitfalls commonly observed. The preparation of IGF-I to be used as radioligand or standard has to be selected carefully since some IGF-I preparations are contaminated with variants which demonstrate different potencies for different antisera used in the RIA. Accurate assessment of IGF-I levels in blood plasma requires an efficient extraction method for the IGF-binding proteins (IGFBPs). Extraction methods to remove the influence of IGFBPs in the RIA were compared using blood plasma of considerable differences in IGF-I/IGFBP ratios. Acidification of plasma before column chromatography on Sephadex G-75 (G75) is generally considered to be the most reliable extraction method, but it is very time-consuming. The acid-ethanol extraction (AE) of plasma is not valid in many situations. Non-parallel displacement to the IGF-I standard was observed with AE-extracted plasma samples in the RIA. In addition, a comparison of IGF-I values obtained in the RIA after AE or G75 extraction of fetal ovine plasma has shown no significant correlation. We report an extraction technique based on a modified AE extraction followed by cryo-precipitation (AEC). AEC extraction on blood plasma reduced residual IGFBPs to a level that did not interfere in the assay. Furthermore, AEC-extracted plasma samples showed parallel displacement in the RIA to highly purified preparations of authentic IGF-I. We observed high correlations, with a slope close to unity, of IGF-I values obtained in the RIA using the AEC or G75 extraction for plasma from different species including adult and fetal sheep, rat, mouse and man. The AEC extraction provides a rapid and simple alternative to G75 extraction for blood plasma from a variety of species provided that high-affinity antisera are used for the RIA.


This article has been cited by other articles: (Search Google Scholar for Other Citing Articles)


Home page
Proc. Natl. Acad. Sci. U. S. A.Home page
G. Keller, A. V. Schally, K. Groot, G. L. Toller, A. Havt, F. Koster, P. Armatis, G. Halmos, M. Zarandi, J. L. Varga, and J. B. Engel
Effective treatment of experimental human non-Hodgkin's lymphomas with antagonists of growth hormone-releasing hormone
PNAS, July 26, 2005; 102(30): 10628 - 10633.
[Abstract] [Full Text] [PDF]


Home page
J Clin Endocrinol MetabHome page
J. B. Engel, G. Keller, A. V. Schally, G. L. Toller, K. Groot, A. Havt, P. Armatis, M. Zarandi, J. L. Varga, and G. Halmos
Inhibition of Growth of Experimental Human Endometrial Cancer by an Antagonist of Growth Hormone-Releasing Hormone
J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3614 - 3621.
[Abstract] [Full Text] [PDF]


Home page
J Anim SciHome page
B. C. Peterson, G. C. Waldbieser, and L. Bilodeau
Effects of recombinant bovine somatotropin on growth and abundance of mRNA for IGF-I and IGF-II in channel catfish (Ictalurus punctatus)
J Anim Sci, April 1, 2005; 83(4): 816 - 824.
[Abstract] [Full Text] [PDF]


Home page
ReproductionHome page
C Vinoles, M Forsberg, G B Martin, C Cajarville, J Repetto, and A Meikle
Short-term nutritional supplementation of ewes in low body condition affects follicle development due to an increase in glucose and metabolic hormones
Reproduction, March 1, 2005; 129(3): 299 - 309.
[Abstract] [Full Text] [PDF]


Home page
Pediatr ResHome page
M. H. OLIVER, B. H. BREIER, P. D. GLUCKMAN, and J. E. HARDING
Birth Weight Rather Than Maternal Nutrition Influences Glucose Tolerance, Blood Pressure, and IGF-I Levels in Sheep
Pediatr. Res., September 27, 2002; 52(4): 516 - 524.
[Abstract] [Full Text] [PDF]


Home page
Clin ChemHome page
J. van Doorn, C. M. Hoogerbrugge, J. G. Koster, R. J. Bloemen, K. Hoekman, A. H. Mudde, and S. C. van Buul-Offers
Antibodies Directed against the E Region of Pro-Insulin-like Growth Factor-II Used to Evaluate Non-Islet Cell Tumor-induced Hypoglycemia
Clin. Chem., October 1, 2002; 48(10): 1739 - 1750.
[Abstract] [Full Text] [PDF]


Home page
Diabetes CareHome page
D. Simmons and B. H. Breier
Fetal Overnutrition in Polynesian Pregnancies and in Gestational Diabetes May Lead to Dysregulation of the Adipoinsular Axis in Offspring
Diabetes Care, September 1, 2002; 25(9): 1539 - 1544.
[Abstract] [Full Text] [PDF]


Home page
J Clin Endocrinol MetabHome page
P. U. Freda
Somatostatin Analogs in Acromegaly
J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3013 - 3018.
[Full Text] [PDF]


Home page
Am. J. Clin. Nutr.Home page
B. Dawson-Hughes and S. S Harris
Calcium intake influences the association of protein intake with rates of bone loss in elderly men and women
Am. J. Clinical Nutrition, April 1, 2002; 75(4): 773 - 779.
[Abstract] [Full Text] [PDF]


Home page
J. Nutr.Home page
M. Katsumata, S. Kawakami, Y. Kaji, R. Takada, and M. J. Dauncey
Differential Regulation of Porcine Hepatic IGF-I mRNA Expression and Plasma IGF-I Concentration by a Low Lysine Diet
J. Nutr., April 1, 2002; 132(4): 688 - 692.
[Abstract] [Full Text] [PDF]


Home page
Pediatr ResHome page
F. H. BLOOMFIELD, B. H. BREIER, and J. E. HARDING
Fate of 125I-IGF-I Administered into the Amniotic Fluid of Late-Gestation Fetal Sheep
Pediatr. Res., March 1, 2002; 51(3): 361 - 369.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol.Home page
F. H. Bloomfield, M. K. Bauer, P. L. Van Zijl, P. D. Gluckman, and J. E. Harding
Amniotic IGF-I supplements improve gut growth but reduce circulating IGF-I in growth-restricted fetal sheep
Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E259 - 269.
[Abstract] [Full Text] [PDF]


Home page
JACCHome page
Y. Iwanaga, Y. Kihara, T. Yoneda, T. Aoyama, and S. Sasayama
Modulation of in vivo cardiac hypertrophy with insulin-like growth factor-1 and angiotensin-converting enzyme inhibitor: relationship between change in myosin isoform and progression of left ventricular dysfunction
J. Am. Coll. Cardiol., August 1, 2000; 36(2): 635 - 642.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
V. Chandrashekar, A. Bartke, C. A. Awoniyi, C. H. Tsai-Morris, M. L. Dufau, L. D. Russell, and J. J. Kopchick
Testicular Endocrine Function in GH Receptor Gene Disrupted Mice
Endocrinology, August 1, 2001; 142(8): 3443 - 3450.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
L. Wang and M. L. Adamo
Cyclic Adenosine 3',5'-Monophosphate Inhibits Insulin-Like Growth Factor I Gene Expression in Rat Glioma Cell Lines: Evidence for Regulation of Transcription and Messenger Ribonucleic Acid Stability
Endocrinology, July 1, 2001; 142(7): 3041 - 3050.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
W. Kwong, A. Wild, P Roberts, A. Willis, and T. Fleming
Maternal undernutrition during the preimplantation period of rat development causes blastocyst abnormalities and programming of postnatal hypertension
Development, January 10, 2000; 127(19): 4195 - 4202.
[Abstract] [PDF]


Home page
J Clin Endocrinol MetabHome page
I. Chatzistamou, A. V. Schally, J. L. Varga, K. Groot, P. Armatis, R. Busto, and G. Halmos
Antagonists of Growth Hormone-Releasing Hormone and Somatostatin Analog RC-160 Inhibit the Growth of the OV-1063 Human Epithelial Ovarian Cancer Cell Line Xenografted into Nude Mice
J. Clin. Endocrinol. Metab., May 1, 2001; 86(5): 2144 - 2152.
[Abstract] [Full Text]


Home page
J. Nutr.Home page
R. J. Hunter, V. B. Patel, J. P. Miell, H. J. Wong, J. S. Marway, P. J. Richardson, and V. R. Preedy
Diarrhea Reduces the Rates of Cardiac Protein Synthesis in Myofibrillar Protein Fractions in Rats In Vivo
J. Nutr., May 1, 2001; 131(5): 1513 - 1519.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
P. D. Raposinho, E. Castillo, V. D’alleves, P. Broqua, F. P. Pralong, and M. L. Aubert
Chronic Blockade of the Melanocortin 4 Receptor Subtype Leads to Obesity Independently of Neuropeptide Y Action, with No Adverse Effects on the Gonadotropic and Somatotropic Axes
Endocrinology, December 1, 2000; 141(12): 4419 - 4427.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
M. J. Toth, A. Tchernof, C. J. Rosen, D. E. Matthews, and E. T. Poehlman
Regulation of Protein Metabolism in Middle-Aged, Premenopausal Women: Roles of Adiposity and Estradiol
J. Clin. Endocrinol. Metab., April 1, 2000; 85(4): 1382 - 1387.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
J. Khosravi, A. Diamandi, J. Mistry, and R. G. Krishna
The High Molecular Weight Insulin-Like Growth Factor-Binding Protein Complex: Epitope Mapping, Immunoassay, and Preliminary Clinical Evaluation
J. Clin. Endocrinol. Metab., August 1, 1999; 84(8): 2826 - 2833.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
E. S. Kurland, F. K. W. Chan, C. J. Rosen, and J. P. Bilezikian
Normal Growth Hormone Secretory Reserve in Men with Idiopathic Osteoporosis and Reduced Circulating Levels of Insulin-Like Growth Factor-I
J. Clin. Endocrinol. Metab., July 1, 1998; 83(7): 2576 - 2579.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
N. Buchs, J.-P. Bonjour, and R. Rizzoli
Renal Tubular Reabsorption of Phosphate Is Positively Related to the Extent of Bone Metastatic Load in Patients with Prostate Cancer
J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1535 - 1541.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
E. S. Kurland, C. J. Rosen, F. Cosman, D. McMahon, F. Chan, E. Shane, R. Lindsay, D. Dempster, and J. P. Bilezikian
Insulin-Like Growth Factor-I in Men with Idiopathic Osteoporosis
J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 2799 - 2805.
[Abstract] [Full Text]


Home page
J Clin Endocrinol MetabHome page
J. Rabinovici, N. A. Cataldo, P. Dandekar, S. M. Rosenthal, S. E. Gargosky, N. Gesundheit, and M. C. Martin
Adjunctive Growth Hormone during Ovarian Hyperstimulation Increases Levels of Insulin-Like Growth Factor Binding Proteins in Follicular Fluid: A Randomized, Placebo-Controlled, Cross-Over Study
J. Clin. Endocrinol. Metab., April 1, 1997; 82(4): 1171 - 1176.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
V. Chandrashekar, A. Bartke, K. T. Coschigano, and J. J. Kopchick
Pituitary and Testicular Function in Growth Hormone Receptor Gene Knockout Mice
Endocrinology, March 1, 1999; 140(3): 1082 - 1088.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
V. Locatelli, G. Rossoni, F. Schweiger, A. Torsello, V. De Gennaro Colonna, M. Bernareggi, R. Deghenghi, E. E. Müller, and F. Berti
Growth Hormone-Independent Cardioprotective Effects of Hexarelin in the Rat
Endocrinology, September 1, 1999; 140(9): 4024 - 4031.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
E. Svanberg, L. Powell-Braxton, C. Ohlsson, H. Zachrisson, and K. Lundholm
The Role of the Growth Hormone/Insulin-Like Growth Factor I Axis in Stimulation of Protein Synthesis in Skeletal Muscles Following Oral Refeeding
Endocrinology, December 1, 1998; 139(12): 4906 - 4910.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
S. Zaina, R. V. S. Newton, M. R. Paul, and C. F. Graham
Local Reduction of Organ Size in Transgenic Mice Expressing a Soluble Insulin-Like Growth Factor II/Mannose-6-Phosphate Receptor
Endocrinology, September 1, 1998; 139(9): 3886 - 3895.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
V. Chandrashekar and A. Bartke
The Role of Growth Hormone in the Control of Gonadotropin Secretion in Adult Male Rats
Endocrinology, March 1, 1998; 139(3): 1067 - 1074.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
M. Tzanela, C. Wagner, and G. Shaffer Tannenbaum
Recombinant Human Growth Hormone-Binding Protein Fails to Enhance the in Vivo Bioactivity of Human Growth Hormone in Normal Rats
Endocrinology, December 1, 1997; 138(12): 5316 - 5324.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
J. E. Harding, P. C. Evans, and P. D. Gluckman
Maternal Growth Hormone Treatment Increases Placental Diffusion Capacity But Not Fetal or Placental Growth in Sheep
Endocrinology, December 1, 1997; 138(12): 5352 - 5358.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
C. Heinrichs, M. Colli, J. A. Yanovski, L. Laue, N. A. Gerstl, A. D. Kramer, J. A. Uyeda, and J. Baron
Effects of Fasting on the Growth Plate: Systemic and Local Mechanisms
Endocrinology, December 1, 1997; 138(12): 5359 - 5365.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
B. Tönshoff, D. R. Powell, D. Zhao, S. K. Durham, M. E. Coleman, H. M. Domené, W. F. Blum, R. C. Baxter, L. C. Moore, and F. J. Kaskel
Decreased Hepatic Insulin-Like Growth Factor (IGF)-I and Increased IGF Binding Protein-1 and -2 Gene Expression in Experimental Uremia
Endocrinology, March 1, 1997; 138(3): 938 - 946.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
E. Melián, B. Velasco, R. Barrios, and F. Sanchez-Franco
Basal and Growth Hormone-Induced Hepatic Messenger Ribonucleic Acid Expression of Insulin-Like Growth Factor-I (IGF-I) and IGF-Binding Protein-3 Is Independent of Hyperinsulinemia and Increased Energy Status in the Genetically Obese Zucker Rat
Endocrinology, March 1, 1997; 138(3): 1066 - 1071.
[Abstract] [Full Text]


Home page
EndocrinologyHome page
P. A. Piosik, M. van Groenigen, J. van Doorn, F. Baas, and J. J. M. de Vijlder
Effects of Maternal Thyroid Status on Thyroid Hormones and Growth in Congenitally Hypothyroid Goat Fetuses during the Second Half of Gestation
Endocrinology, January 1, 1997; 138(1): 5 - 11.
[Abstract] [Full Text]


Home page
Am. J. Physiol.Home page
K. D. Laugero and G. P. Moberg
Energetic response to repeated restraint stress in rapidly growing mice
Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E33 - 43.
[Abstract] [Full Text]


Home page
Am. J. Physiol.Home page
K. D. Laugero and G. P. Moberg
Summation of behavioral and immunological stress: metabolic consequences to the growing mouse
Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E44 - 49.
[Abstract] [Full Text]


Home page
J. Appl. Physiol.Home page
G. R. Adams, S. A. McCue, P. W. Bodell, M. Zeng, and K. M. Baldwin
Effects of spaceflight and thyroid deficiency on hindlimb development. I. Muscle mass and IGF-I expression
J Appl Physiol, March 1, 2000; 88(3): 894 - 903.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. U. S. A.Home page
H. Kiaris, A. V. Schally, J. L. Varga, K. Groot, and P. Armatis
From the Cover: Growth hormone-releasing hormone: An autocrine growth factor for small cell lung carcinoma
PNAS, December 21, 1999; 96(26): 14894 - 14898.
[Abstract] [Full Text] [PDF]


Home page
J. Appl. Physiol.Home page
G. R. Adams, F. Haddad, and K. M. Baldwin
Time course of changes in markers of myogenesis in overloaded rat skeletal muscles
J Appl Physiol, November 1, 1999; 87(5): 1705 - 1712.
[Abstract] [Full Text]


Home page
Am. J. Physiol.Home page
M. Pendergrass, E. Fazioni, D. Collins, and R. A. DeFronzo

Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E345 - 350.
[Abstract]


Home page
Proc. Natl. Acad. Sci. U. S. A.Home page
N. Lamharzi, A. V. Schally, M. Koppan, and K. Groot
Growth hormone-releasing hormone antagonist MZ-5-156 inhibits growth of DU-145 human androgen-independent prostate carcinoma in nude mice and suppresses the levels and mRNA expression of insulin-like growth factor II in tumors
PNAS, July 21, 1998; 95(15): 8864 - 8868.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol.Home page
R. M. Sapolsky and E. M. Spencer
Insulin-like growth factor I is suppressed in socially subordinate male baboons
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1346 - 1351.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
C. E. Perrone, D. Fenwick-Smith, and H. H. Vandenburgh
Collagen and Stretch Modulate Autocrine Secretion of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Proteins from Differentiated Skeletal Muscle Cells
J. Biol. Chem., February 3, 1995; 270(5): 2099 - 2106.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Invest.Home page
M. Brink, J. Wellen, and P. Delafontaine
Angiotensin II Causes Weight Loss and Decreases Circulating Insulin-like Growth Factor I in Rats through a Pressor-independent Mechanism
J. Clin. Invest., June 1, 1996; 97(11): 2509 - 2516.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. U. S. A.Home page
A. Jungwirth, A. V. Schally, J. Pinski, K. Groot, P. Armatis, and G. Halmos
Growth hormone-releasing hormone antagonist MZ-4-71 inhibits in vivo proliferation of Caki-I renal´┐Żadenocarcinoma
PNAS, May 27, 1997; 94(11): 5810 - 5813.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. E. H. Stewart and P. Rotwein
Insulin-like Growth Factor-II Is an Autocrine Survival Factor for Differentiating Myoblasts
J. Biol. Chem., May 10, 1996; 271(19): 11330 - 11338.
[Abstract] [Full Text] [PDF]




HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the Society for Endocrinology.