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333MMM` f` f` 3?l-] } l-]} l-]} l-] } l-] ?" d]D } -K] k} l-]0} -K]@} l-]P ?" dd  }  dd  } " dd@ }  dd` } dd @?l-] } l-]} l-]} l-] } l-] P}l-] }l-] }l-] }l-]  }l-]  `}l-]}l-]}l-]}l-] }l-] p}" dd } dd }" dd@} dd`}dd}" dd } dd }" dd@} dd`}dd ld(    Z$0z   zFClick to edit the title text format$ $  Z0z ,  Click to edit the outline text format Second Outline Level Third Outline Level Fourth Outline Level Fifth Outline Level Sixth Outline Level Seventh Outline Level Eighth Outline Level Ninth Outline Level6& N  6qљYs ? ̙33L   (    Z1z z6     Zd2z  81  H  0޽h ? ̙33 % 800 (     T2z ? ) ~Figure 2 ER structure, post-translational modifications and conformational changes induced by different ligands. .sl-]s s 6 X  x @`j  BA ?1mj  BA ?t\f  T$3z ?3t fT Traboulsi et al. J Mol Endocrinol 2017;58:R15-R31,4l-]4  4 X .  T3z ?D Z $ 2017 The authors*l-]   & X  x 6N  6qљYs ? ̙33ZR___PPT102.]0G+D' = @B D' = @BA?%,( < +O%,( < ++>  5   @z (  @  C ?z7   T3z ? 92   ^`ER structure, post-translational modifications and conformational changes induced by different ligands. (A) Schematic representation of ER structure. AF1/AF2: activation function 1/2; DBD: DNA-Binding Domain; NLS: Nuclear Localization Signal; LBD: Ligand-Binding Domain. SUMOylation sites identified by mass spectrometry in the presence of ICI 182,780 are indicated in purple. Residues phosphorylated in the presence of antiestrogens or implicated in the modulation of sensitivity to antiestrogen treatment are indicated in orange. (B) LBD ER  estradiol (E2)  TIF2 NR box 3 complex (Warnmark et al. 2002); (C) LBD ER  4-hydroxytamoxifen complex (OHT) (Shiau et al. 1998); (D) LBD ER  ICI 164,384 complex (Pike et al. 2001); (E) LBD ER  GW5638 complex (Wu et al. 2005). Representations were generated using PyMOL. Helix 12 is highlighted in red and each ligand is shown in green. The -helical TIF2 coactivator motif is shown in gold.0 l-]U# # ?# # 1# # *# # #   & X  x 6H  0޽h ? ̙33rP> ^| (Root EntrydO)CompObj9Ole Current User,Pictures5SummaryInformation(QxPowerPoint Document()DocumentSummaryInformation8